[Phys-seminars] 2017-12-07 Lasers Seminar
barmash at bgu.ac.il
Sat Dec 2 17:09:43 IST 2017
PLACE: Physics building (#54) room 207
Using Photon Statistics to Understand Microsecond Dynamics of Proteins
Gilad Haran, Chemical Physics Department, Weizmann Institute of Science
Single-molecule fluorescence spectroscopy is a method of choice for studying the mechanism of action of complex biological machines in real time. In particular, single-molecule fluorescence resonance energy transfer (smFRET), where the interaction between two fluorescent molecules is probed, has been shown to yield important insights on protein dynamics. We recently developed a novel photon-by-photon analysis method that pushes the time resolution of smFRET to the microsecond time scale. In this lecture I will briefly describe how photon statistics can be used to obtain information on protein motions, and then show how we apply it to important biological problems.
More information about the Phys-seminars